Differences in physicochemical properties of pectin extracted from pomelo peel with different extraction techniques

In order to obtain high yield pomelo peel pectin with better physicochemical properties, four pectin extraction methods, including hot acid extraction (HAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction, and enzymatic assisted extraction (EAE) were compared. MAE led to the highest pectin yield (20.43%), and the lowest pectin recovery was found for EAE (11.94%). The physicochemical properties of pomelo peel pectin obtained by different methods were also significantly different. Pectin samples obtained by MAE had the highest methoxyl content (8.35%), galacturonic acid content (71.36%), and showed a higher apparent viscosity, thermal and emulsion stability. The pectin extracted by EAE showed the highest total phenolic content (12.86%) and lowest particle size (843.69 nm), showing higher DPPH and ABTS scavenging activities than other extract methods. The pectin extracted by HAE had the highest particle size (966.12 nm) and degree of esterification (55.67%). However, Fourier-transform infrared spectroscopy showed that no significant difference occurred among the different methods in the chemical structure of the extracted pectin. This study provides a theoretical basis for the industrial production of pomelo peel pectin.

oil.Pomelo peel is a potential source of pectin 14 .However, pomelo peel has not been exploited as a pectin resource and is usually discarded as garbage.Only a few studies have investigated the physicochemical properties of pomelo pectin, and the optimal extraction method of pomelo pectin has not been considered.Besides, it is clear that the influences of different extracted methods on physicochemical properties of pomelo peel pectin.The structure of pectin depends on the plant source and the method of extraction, which is a determinant factor on its physicochemical properties and application 15 .Therefore, this study compared HAE, MAE, UAE, and EAE in terms of obtained yield and physiochemical properties in pomelo peel.The comparisons of results are helpful to associations of physiochemical properties and extraction methods, and offer a basis in the industrial production of pomelo peel pectin.

Materials
Pomelo fruits of the same batch and ripeness were obtained from Guangzhou, China.The peels were cut and separated from the flesh.Subsequently, the peels were dried in an oven at 60 °C until no change was observed in the weight.The dried peels were ground and sieved (250-300 μm) to obtain pomelo peel powder.All reagents and chemicals used in this study were of analytical grade and supplied by Sigma-Aldrich, St. Louis, Missouri, USA.

Pectin extraction
(1) Hot acid extraction (HAE): HAE of pectin was carried out based on the method of Torralbo et al. 16 with minor modification.Pomelo peel powder (10 g) was mixed with 0.1 M water-citric acid solution (100 mL; 2.0 pH), and then the mixture was kept in a water bath at 90 °C with continual agitation for 60 min.
After each extraction, the mixture was centrifuged (5000 g, 10 min), and the supernatant was collected.Then, the supernatant was precipitated by adding ethyl alcohol (2:1, v/v) and kept at 4 °C for 12 h.The precipitated pectin was collected, and all the extractions were performed in triplicate.Finally, the pectin was freeze dried and stored at ambient conditions for further analyses.
(2) Microwave-assisted extraction (MAE): MAE of pectin was carried out based on the method described by Li et al. 17 .Pomelo peel powder (10 g) was mixed with 0.1 M water-citric acid solution (100 mL; 2.0 pH) in a beaker.The mixing was performed in an experimental microwave oven (power of 50 W, frequency of 2450 MHz, PreeKem Scientific Instruments Co., Ltd.China) at 100 °C for 4 min.The other steps were kept the same as that of HAE.(3) Ultrasound-assisted extraction (UAE): UAE of pectin was carried out based on the method described by Wang et al. 18 with slight modification.Pomelo peel powder (10 g) was mixed with 0.1 M water-citric acid solution (100 mL; 2.0 pH).The mixture was sonicated for 20 min by an ultrasonic device (ultrasound intensity, 480.2 W/cm 2 ; temperature, 45 °C) and stirred continuously.The other steps were kept the same as that of HAE.(4) Enzyme-assisted extraction (EAE): Pomelo peel powder (10 g) was mixed with water-citric acid solution (100 mL; 4.5 pH).Then, the extraction process began by adding 2 g of cellulase enzyme (5000 U/g, food grade, Imperial Jade Bio-technology Co., Ltd.) to the mixture, followed by conducting the extraction at 47 °C for 10 h with constant shaking at 200 rpm.After the extraction, the samples were heated at 90 °C for 5 min to inactivate the enzyme.Afterward, the samples were cooled 19 .All other steps remained the same as the HAE.

Extraction yield and particle size
The extraction yields (EY, %) of the pectin obtained were calculated by the Eq.(1): The particle size of the pectin was measured using particle size analyser (Malvern, UK) based on a previous report 20 .

Chemical composition analysis
Protein content was determined by the Bradford method with bovine serum albumin as the standard 21 .The total phenolic content was calculated using the Folin-Ciocalteu method as described by Hosseini et al. 22 .The methoxyl content of the pectin was calculated based on the previous report 23 , The galacturonic acid content was measured with the m-hydroxydiphenyl method described by Dranca et al. 13 .The degree of esterification (DE) of the pectin sample was measured based on a previous study 24 .

Fourier-transform infrared spectroscopy (FTIR)
Pectin samples were mixed with KBr (1:100) and pressed into pellets before the measurement.FTIR spectra was determined using a Nicolet 5700 FTIR spectrometer (Thermo Fisher Scientific, USA) in the wavenumber range of 400-4000 cm −1 at a resolution of 4 cm −1 .

Rheological properties
The rheological property was investigated with AR1500ex Rheometer (TA Instruments, New Castle, USA) with a 50 mm parallel plate according to a previous study.The pectin sample 1% (w/w) was dissolved in distilled water.
The Rheological properties analysis of pectin was conducted under a shear rate from.

Antioxidant capacity
DPPH radical scavenging activity was measured based on a previous report 26 .Briefly, the sample (2.5 mL) was mixed with 1.5 mL DPPH solution (100 mM).After 20 min, the absorbance was measured at a wavelength 517 nm using a spectrophotometer.The DPPH radical scavenging activity was calculated by the Eq. ( 4): where A 0 and A n are the absorbance values of the control and test samples, respectively.ABTS radical scavenging activity was measured using a kit (Nanjing Jiancheng Bioengineering Institute Co., Ltd.).

Data analysis
All assays and tests were performed in triplicate, and the results were expressed as means ± standard deviation.The significance of the difference was analyzed by Duncan's multiple range test with SPSS (Version 17.0).Graphs were generated using OriginPro 2022b.

Extraction yield and particle size by different methods
Table 1 shows that MAE (20.43%) and UAE (17.21%) achieved higher extraction yields of pectin from pomelo peel.The results were significantly (p < 0.05) higher than those of pectin extracted by HAE (15.36%).This result is in agreement with the results obtained by Jiang et al. on pectin extraction from seed watermelon peel 27 .This phenomenon may be due to microwaves weakening the cell wall structure and causing parenchymal cells to cleave, increasing the contact between the solvent and the extracting substance 28 .However, the extraction yield of EAE (11.94%) was the lowest, which was significantly lower than that of HAE (p < 0.05).Similarly, Bagherian et al. 11 reported that MAE (27.81%) resulted in higher pectin extraction yield from grapefruit than that of HAE (19.16%).The higher extraction yield of MAE can be attributed to microwave radiation, which causes the plant tissue rupture and cell wall matrix loosening 29 .Contrary to the results of previous studies 30 , EAE did not (2) EA(%) = the volume of the emulsified layer(mL) the whole volume of the mixture(mL) × 100%, the volume of the remaining emulsified layer after 30 days(mL) the volume of the emulsified layer(mL) × 100%, www.nature.com/scientificreports/produce a pectin yield higher than that of HAE.This result may be primarily because available enzymes cannot wholly hydrolyze plant cell walls, and enzymic response depends on some factors (e.g., reaction time, type and concentration of enzyme, temperature) 31 .Additionally, the solid-to-liquid ratio was the same (1:10) for HAE, UAE, MAE, and EAE.However, the extraction time was considerably shorter for MAE (4 min) and UAE (20 min) than for HAE (60 min).The pectin extracted by MAE (881.31nm), UAE (918.75 nm), and EAE (843.69 nm) had a relative lower particle size than that extracted by HAE (966.12 nm) (Table 1).The effects of HAE, MAE, and UAE on pectin particle size were similar to those previously reported 32 from the comparison of pectin extracted from black carrot pomace using HAE, MAE, and UAE.In another study, pectin particle size affected pectin's emulsifying properties, which is important for pectin usage in food 33 .

Chemical composition of pectin by different methods
As shown in Table 1, there was a significant difference (p ˂ 0.05) in the protein content of the pectin extracted using different methods.The highest protein content was found in the pectin extracted by EAE (12.86%).The pectin of UAE (9.32%) had a lower protein content compared to HAE (10.34%) possibly due to the hydrolysis of protein caused by ultrasound and papain, leading to a decrease in protein content 30 .Although the pectin content of the MAE (9.06%) extraction method was lower compared to other methods, this disparity may be attributed to the covalent bond formed between the amino group on the protein's side chain and the terminal hydroxyl groups in the polysaccharides via the Maillard reaction 34 .
Compared with HAE, EAE and UAE displayed higher total phenolic content in pectin (Table 1).The cell wall's structural integrity may be weakened by enzymes and ultrasonic methods, leading to the release of polyphenols from pomelo peel.This occurs while minimizing or reducing the structural alterations of polyphenols at lower temperatures.Whereas, HAE and MAE needed a higher temperatures (> 80 °C), avoiding or decreasing structural changes of polyphenols and decreased the total phenolic content in pectin 13 .This result was consistent with previous results reported by Bai et al. 28 that the potato polysaccharide obtained by EAE or UAE has higher total phenolic content.
The gel capacity of pectin is significantly influenced by the methoxyl content.As shown in Table 1, the methoxyl content of pectin obtained by MAE (8.35%) was significantly higher (p < 0.05) than other extraction methods, which is similar to the result of pectin extracted from kinnow peel 23 .The elevated methoxyl concentration in pectin obtained via MAE could be ascribed to the existence of an esterified carboxyl group 35 .According to the report, pectin with a higher methoxyl content is more soluble in water than pectin with a lower methoxyl content 36 .The lowest methoxyl content was observed in the pectin extracted from EAE (6.86%).The methoxyl content of UAE and HAE was 7.43% and 7.28%, which is similar to the result of pectin extracted from lime peel 37 .The methoxyl content of pectin was not significant difference among the UAE, EAE and HAE.Commercial pectin typically contains 8-11% methoxyl content and can form high sugar gels exceeding 65%.In contrast, the presence of low methoxyl pectin allows for the generation of gels containing sugar levels below 7.0% 36 .
The galacturonic acid is regarded as the backbone of the pectin molecule, and the content of galacturonic acid could determine the purity of pectin 18 .Pectin extracted by MAE (71.36%) and UAE (69.62%) had a higher galacturonic acid content than that extracted by HAE (Table 1).The galacturonic acid content of pectin extracted from lime peel using MAE was higher than the HAE 34 .Similar results have been shown by the pectin extracted from sisal waste using UEA 38 .According to previous reports that MAE and UAE have a superior capability to entirely release pectic compounds with more galacturonic acid from deeper parts of the plant structure 38 .However, for EAE pectin (56.34%), the galacturonic acid content was lower than that of the HAE pectin (67.36%).Similar values were observed from the date and lemon pectin (41.5 to 74.5%) which were extracted using acidified water.The content was consistent with the extracted yield of pectin extracted by different methods.According to the FAO and EU requirements, the galacturonic acid content in industrial pectin should be at least 65% 39 .Therefore, the pectin extracted by EAE is unsuitable for forming strong gels in the food industry.
The DE had a significant impact on the functional properties of pectin.Table 1 indicates that the DE of pectin obtained through HAE (55.67%) surpassed that of pectin obtained through alternative techniques.Nevertheless, EAE achieved the lowest DE of pectin (47.71%), significantly inferior to that attained by HAE.The DE can be categorized as low methoxyl pectin due to its concentration falling below 50% 40 .Additionally, the DE of pectin extracted by MAE (53.34%) and UAE (51.42%) was lower than that extracted by HAE.These results are consistent with previous studies, showing that the DE of pectin obtained by UAE and MAE was lower than that obtained by HAE 41 .The pectin extracted through MAE and UAE is subjected to harsh conditions, which leads to higher de-esterification of polygalacturonic chains, resulting in a decreased DE 11,42 .

FTIR
Figure 1 illustrates the FTIR spectra of the pectin obtained by different extraction methods.The pectin extracted by the different methods exhibited similar FTIR spectra, showing that the main structure of the pectin was not influenced by the different extraction methods, further confirming that the extracts were pectin.For all the pectin samples, the wide and strong absorption at 3500 cm −1 was caused by the stretching of oxygen-hydrogen bond (-OH), and the absorption peak at 2936 cm −1 was attributed to the C-H bond (including CH, CH 2 , and CH 3 ) vibration (Table 2) 43 .Meanwhile, the bonds observed in the regions from 1735 to 1750 cm −1 and 1600 to 1400 cm −1 were identified as the esterified carboxyl (-COOR) and free carboxylic groups (-COO − ), respectively.The absorption bands at 1010 cm −1 indicate that the existence of pyranose.According to the FTIR spectra, a polysaccharide-rich polygalacturonic acid was the pectin extracted from the pomelo peel using different methods, and the results were in agreement with those of pectin extracted from jackfruit rags 27 .Additionally, the pectin FTIR patterns extracted with the different methods were similar.

Thermal properties
Figure 2 shows that apparent endothermic peaks (melting temperature) did not occur.The endothermic peaks resulted from water evaporation, and our results are consistent with those of Qin et al. 25 , showing that no bound water was removed from the pectin samples.All the pectin samples showed exothermic peaks between 240 and 265 °C, where the pectin degradation began.The maximum exothermic peaks for CHE, MAE, UAE, and EAE were at 246.2, 260.1, 244.7, and 242.9 °C, respectively.The pectin obtained by MAE had a broader exothermic   www.nature.com/scientificreports/peak, indicating that the pectin had a higher thermal stability.Additionally, the pectin obtained by HAE had a broader exothermic peak than that of other methods, indicating its wider molecular weight distribution, which correlates with molecular weight distribution 13 .

Emulsifying properties
Pectin is utilized as a stabilizer or emulsifier in foods because of its emulsifying properties.The emulsifying properties of pectin extracted by the four methods are presented in Fig. 4, including EA and ES.The EAs of the pectin extracted by the four methods was no significant difference (p > 0.05) (Fig. 3A).However, the pectin extracted by HAE had the lowest ES, which was significantly lower (p < 0.05) than those extracted by other methods (Fig. 3B).These results are consistent with those of a previous study, showing that UAE increased the galacturonic acid content and caused increased emulsifying properties 22 , compared with those of HAE.The molecular weight, galacturonic acid content, and particle size significantly influenced the emulsifying properties of the extracted pectin 44 .In this study, the pectin extracted by UAE and MAE had higher galacturonic acid content and lower particle size, which increased the emulsifying properties.

Rheological properties analysis of pectin
The pectin solution samples, as shown in Fig. 4, showed a decrease in viscosity and an increase in shear rate, suggesting that the fluid was pseudoplastic.The gradual weakening of pectin's intermolecular strength during the test may account for the observed shear-thinning trend across all the curves.The shear-thinning behavior is a result of the pectin's intermolecular forces decreasing as the shear rate increases 45 .Apparently, the viscosity of pectin extracted by MAE was higher than that of the other methods.This result is consistent with the findings of Chen et al. 46 , who reported that the pectin extracted by MAE had higher molecular weight and weakened relative motion of molecules, and thus showed a high apparent viscosity.However, the pectin extracted by UAE retained low and stable viscosity.

Antioxidant activities
Pectin has antioxidant activity due to the hydroxyl group of the molecule 47 .The colour of samples changes from purple to yellow when the free radicals of DPPH are scavenged by antioxidant compounds 48 .After the reaction between ABTS• + and an antioxidant, ABTS• + is converted to its non-radical form by donating an electron 43 .Therefore, the DPPH and ABTS radical scavenging activities are widely used in measuring antioxidant activities 49,50 .As shown in Fig. 5A,B, all the pectin with different extraction showed DPPH and ABTS radical scavenging activities, showing the antioxidant activities positively correlated with their concentration from 0.5 to 2.5 mg/mL.These results are consistent with the results reported by Gharibzahedi et al. 43 .However, it is difference that the antioxidant properties of pectin obtained by different extraction methods.These results indicated a significant correlation between the antioxidant activities and extraction methods.At same concentration, the pectin extracted by EAE had the highest DPPH and ABTS radical scavenging activities.The results are consistent with the findings of Wang et al. 1 , who reported that extracted apple pectin had considerably higher antioxidant activity than that of HAE.This phenomenon could be attributed that the pectin obtained by EAE displayed higher total phenolic content and lower particle size, which improving more reactive sites with free radicals and increased antioxidant activities 28 .Additionally, the pectin extracted by UAE and MAE had substantially higher DPPH (Fig. 5A) and ABTS (Fig. 5B) radical scavenging activity ranges than that extracted by HAE at same concentration.The higher antioxidant capacity of pectin can be attributed to the higher galacturonic acid content of pectin extracted by UAE and MAE.It was reported by Wang et al. 51 that the antioxidant activity of pectin also depends on the amount of galacturonic acid besides total phenolic content.These findings indicate that by using the right method and parameters, it is possible to produce pectin with potent antioxidant activity.

Conclusions
In this study, HAE, UAE, MAE, and EAE methods were used to extract pectin from pomelo peel, and their effects on pectin physicochemical properties were compared.MAE and EAE resulted in the highest (20.43%) and lowest (11.94%) pectin yield from pomelo peel compared to the other methods.Fourier-transform infrared spectroscopy showed that no significant difference occurred among the different methods in the chemical structure of the extracted pectin.However, the physicochemical properties of pomelo peel pectin obtained by different methods were also significantly different.The pectin extracted by HAE had the highest particle size (966.12nm) and degree of esterification (55.67%).The pectin obtained by MAE had the highest methoxyl content (8.35%), galacturonic acid content (71.36%), and showed a higher apparent viscosity, thermal and emulsion stability.The pectin extracted by EAE showed the highest total phenolic content (12.86%) and lowest particle size (843.69nm), showing higher DPPH and ABTS scavenging activities than other extract methods.Therefore, EAE gained peel pectin with high biological activity, which has good application prospects in the functional-food industries.

Figure 1 .
Figure 1.FT-IR spectra of pectin extracted using different methods.

Figure 3 .
Figure 3. EA (A) and ES (B) of pectin extracted using different methods.Different letters (a, b, c, d) indicate significantly different means at p < 0.05.

Figure 4 .
Figure 4.The apparent viscosity of the pectin extracted using different methods.

Figure 5 .
Figure 5. DPPH (A) and ABTS (B) radical scavenging activities of the pectin extracted by different methods at various concentrations.Different letters (a, b, c, d) indicate significantly different means at p < 0.05.

Table 1 .
Yield and composition of pectin extracted using different methods.

Table 2 .
The summary of FT-IR spectra analysis.